Restriction Enzymes
New England Biolabs, Inc. Fpg – 500 units
Fpg (formamidopyrimidine [fapy]-DNA glycosylase) (also known as 8-oxoguanine DNA glycosylase) acts both as a N-glycosylase and an AP-lyase. The N-glycosylase activity releases damaged purines from double stranded DNA, generating an apurinic (AP site). The AP-lyase activity cleaves both 3' and 5' to the AP site thereby removing the AP site and leaving a 1 base gap. Some of the damaged bases recognized and removed by Fpg include 7, 8-dihydro-8-oxoguanine (8-oxoguanine), 8-oxoadenine, fapy-guanine, methy-fapy-guanine, fapy-adenine, aflatoxin B1-fapy-guanine, 5-hydroxy-cytosine and 5-hydroxy-uracil.
New England Biolabs, Inc. Blue Protein Loading Dye – 8 ml
The blue protein loading dye contains one vial of blue loading buffer and one vial of 30X reducing agent. The combined solution is ideal for protein gel applications.
New England Biolabs, Inc. ScrFI – 1000 units
A restriction endonuclease that recognizes the sequence CC^N_GG.
New England Biolabs, Inc. Endo Hf – 500000 units
Endo Hf is a recombinant protein fusion of Endoglycosidase H and maltose binding protein. Endo Hf cleaves within the chitobiose core of high mannose and some hybrid oligosaccharides from N-linked glycoproteins equally as well as Endo H.
New England Biolabs, Inc. EcoRI – 10000 units
A restriction endonuclease that recognizes the sequence G^AATT_C.
New England Biolabs, Inc. MspI – 25000 units
A restriction endonuclease that recognizes the sequence C^CG_G.
New England Biolabs, Inc. BstXI – 5000 units
A restriction endonuclease that recognizes the sequence CCAN_NNNN^NTGG.
New England Biolabs, Inc. HaeIII – 15000 units
A restriction endonuclease that recognizes the sequence GG_^CC.
New England Biolabs, Inc. HindIII – 50000 units
A restriction endonuclease that recognizes the sequence A^AGCT_T.
New England Biolabs, Inc. HpaI – 2500 units
A restriction endonuclease that recognizes the sequence GTT_^AAC.
New England Biolabs, Inc. EcoRI – 10000 units
A restriction endonuclease that recognizes the sequence G^AATT_C.
New England Biolabs, Inc. HincII – 5000 units
A restriction endonuclease that recognizes the sequence GTY_^RAC.
New England Biolabs, Inc. AatII – 2500 units
A restriction endonuclease that recognizes the sequence G_ACGT^C.
New England Biolabs, Inc. NdeI – 20000 units
A restriction endonuclease that recognizes the sequence CA^TA_TG.
New England Biolabs, Inc. SfiI – 15000 units
A restriction endonuclease that recognizes the sequence GGCCN_NNN^NGGCC.