Restriction Enzymes
New England Biolabs, Inc. HincII – 1000 units
A restriction endonuclease that recognizes the sequence GTY_^RAC.
New England Biolabs, Inc. EcoRV – 4000 units
A restriction endonuclease that recognizes the sequence GAT_^ATC.
New England Biolabs, Inc. XbaI – 3000 units
A restriction endonuclease that recognizes the sequence T^CTAG_A.
New England Biolabs, Inc. BmtI-HF – 1500 units
A high fidelity restriction endonuclease that recognizes the sequence G_CTAG^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. NsiI-HF – 1000 units
A high fidelity restriction endonuclease that recognizes the sequence ATGCA^T. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. SpeI-HF – 2500 units
A high fidelity restriction endonuclease that recognizes the sequence A^CTAGT. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes.
New England Biolabs, Inc. Bsu36I – 1000 units
A restriction endonuclease that recognizes the sequence CC^TNA_GG.
New England Biolabs, Inc. ApaI – 5000 units
A restriction endonuclease that recognizes the sequence G_GGCC^C.
New England Biolabs, Inc. AluI – 1000 units
A restriction endonuclease that recognizes the sequence AG_^CT.
New England Biolabs, Inc. DraI – 2000 units
A restriction endonuclease that recognizes the sequence TTT_^AAA.
New England Biolabs, Inc. NarI – 500 units
A restriction endonuclease that recognizes the sequence GG^CG_CC.
New England Biolabs, Inc. Esp3I – 1500 units
A Type IIS restriction endonuclease that recognizes the sequence CGTCTCN^NNNN_....
New England Biolabs, Inc. SphI-HF – 2500 units
A high fidelity restriction endonuclease that recognizes the sequence GCATG^C. High Fidelity (HF) Restriction Enzymes have 100% activity in rCutSmart Buffer; single-buffer simplicity means more straightforward and streamlined sample processing. HF enzymes also exhibit dramatically reduced star activity. HF enzymes are all Time-Saver qualified and can therefore cut substrate DNA in 5-15 with the flexibility to digest overnight without degradation to DNA. Engineered with performance in mind, HF restriction enzymes are fully active under a broader range of conditions, minimizing off-target products, while offering flexibility in experimental design. NEB extensively performs quality controls on all standard and high-fidelity (HF) restriction enzymes. Examples of nuclease contamination studies for some of our HF restriction enzymes are shown below.
New England Biolabs, Inc. AscI – 500 units
A restriction endonuclease that recognizes the sequence GG^CGCG_CC.
New England Biolabs, Inc. BclI – 3000 units
A restriction endonuclease that recognizes the sequence T^GATC_A.